How Much Time Do You Spend with Off-line Measurements?

On-line parameters like pH and dissolved oxygen are commonly used to optimize process yield. Further optimization is now possible with a new breed of sensors for on-line measurement of viable and total cell density. Data from traditional off-line measurements is limited and time consuming to obtain. The off-line results are delayed and process adjustments are manual. Real-time measurement and control based on cell density is a critical consideration for any modern automated control strategy.


See Specific Applications of On-line Cell Density Monitoring

Learn more how Viable Cell Density Monitoring works for your process in our ebook that features must-see examples of real applications using Hamilton viable cell density monitoring (Incyte) and total cell density monitoring (Dencytee).

Hamilton Process Analytics
Offers a Better Way Forward

Hamilton’s Incyte (viable cell density) and Dencytee (total cell density) sensors bring to life the vision of on-line, real-time measurements of total and viable cell density. With clear, up-to-date information, critical events that could have been missed between off-line samples are now immediately recognizable. That data empowers process engineers to automate production processes and optimize product development.

Use Incyte for On-Line Monitoring of Viable Cell Density

The Incyte sensor enables real-time, on-line measurement of viable cells in solution. The measurement is not influenced by changes in the media, microcarriers, dead cells, or debris. On-line measurement of viable cells makes it possible to detect events and respond in real time without sampling.

  • Increase yield and lower production costs
  • Detect changes in cell physiology with frequency scanning
  • Precisely control harvesting for continuous culturing
  • Early detection of process deviations
  • Sensors optimized for multiple conductivity ranges

How does Hamilton Incyte work?

The Incyte measurement principle is based on capacitance. In an alternating electrical field, viable cells behave like small capacitors. The charge from these small capacitors is measured by the sensor and reported as permittivity (capacitance per area).

Use Dencytee for On-Line Measurement of Total Cell Density

The Dencytee sensor performs on-line measurement of total cell density in solution. The sensor is based on optical density, which measures the turbidity of the cell suspension. The measurement is made at NIR (near-infrared) wavelengths so it is insensitive to changes in media color. All particles and molecules that absorb or scatter light at 880 nm will be detected, including living and dead cells as well as cell debris. This measurement is effective after inoculation, when cells are expanding quickly but concentrations are low, making permittivity-based readings less reliable.

  • Simple on-line measurement of cell growth
  • Reliable values during the growth phase
  • Early detection of process deviations

How does Hamilton Dencytee work?

The Dencytee sensor emits light through a 5 mm window onto a light detector. Cells in suspension absorb and scatter light so less light is read by the detector. To compensate, the sensor increases the amount of light emitted by the light source to maintain a constant reading at the detector. By reading the amount of light that is increased at the light source, the Dencytee sensor can measure solutions with a broad range of cell densities.

Download the Cell Density Applications Ebook

The ebook features must-see examples of real Hamilton Incyte and Dencytee applications and demonstrates how to leverage Viable Cell Density Monitoring in your processes.

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